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KMID : 0380420100340040331
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Journal of Prventive Veterinary Medicine
2010 Volume.34 No. 4 p.331 ~ p.340
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Multi-residues determination of 13 quinolones in pork by liquid chromatograph with tandem mass spectrometry
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Lim Chae-Mi
Kwon Hyun-Jung
Cho Byung-Hoon
Park Su-Jeong
Chung Gab-Soo
Son Seong-Wan
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Abstract
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A reversed-phase high-performance liquid chromatography with tandem mass-spectrometric detection was developed and validated for the simultaneous analysis of 13 quinolones (orbifloxacin, sarafloxacin, marbofloxacin, ofloxacin, enrofloxacin, danofloxacin, pefloxacin, ciprofloxacin, norfloxacin, difloxacin, oxolinic acid, flumequine, nalidixic acid) in pork. The drugs were extracted from homogenized sample using acidic buffer (2% trichloracetic acid) and acetonitrile with ethyl acetate. Analysis was performed by liquid chromatograph with tandem mass spectrometry (LC-MS/MS) in positive mode for all quinolones. Mass spectral acquisition was performed in the multi reaction monitoring mode (MRM), selecting two structurally significant transitions per compound. Good linear relationship (R2=0.999) was observed at 6 concentrations of 2.5～100 ng/g. Satisfied recoveries (73.3～105.4%) of all quinolones were demonstrated in spiked pork at three levels from 5 to 20 ng/g. The limits of quantitation (LOQs) for quinolones ranged from 0.25 to 5.0 ng/g.
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KEYWORD
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Quinolones, LC-MS/MS, Multi-Residues, Pork
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